Figure 2.

Bcl-2 does not increase expression of Ca²⁺-binding proteins. Extracts from untreated cells listed on the horizontal axis were resolved by SDS-PAGE and transferred to nitrocellulose membranes. Lanes 1–4 were subjected to Western blotting using anti-Bcl-2 antibody (lanes 1 and 2) and anticalbindin antibody (lanes 3 and 4) as probes. Lanes 5–8 were incubated with ⁴⁵Ca²⁺ and then exposed to x-ray film. Molecular mass standards are in lane 9. An unidentified protein that immunoreacts with anticalbindin antibody is detected at ∼80-kD in lane 4, and an unidentified Ca²⁺-binding protein was detected in CaBP20 cells (lane 5) midway between the 49.5- and 80-kD markers. The appearance of these proteins was variable among experiments and therefore not considered significant.