Figure 3.

N-cadherin promotes aggregation in BHK cells. BHK cells expressing exogenous N-cadherin (N-cadherin) and control cells (control) were placed in hanging drops for 24 h to promote aggregation. The cells were then collected, placed on slides, and grown for 48 h to permit strong attachment to the slides. During this time N-cadherin– expressing cells remained as aggregates whereas the control cells spread out as a monolayer. Cells were fixed and observed by phase contrast microscopy.