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. 1997 Sep 22;138(6):1323–1331. doi: 10.1083/jcb.138.6.1323

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Nonfunctional N-cadherin increases β-catenin but does not promote skeletal muscle differentiation. Stable cell lines transfected with either pHβ-NcadΔ plus pLKpac (truncated N-cadherin) or pLKpac alone (control) were aggregated and analyzed by immunofluorescence light microscopy as described in Fig. 2. Truncated N-cadherin was detected with 6B3, β-catenin with 15B8, and sarcomeric myosin with MF20. Cells expressing truncated N-cadherin and control cells spread as a monolayer after replating due to lack of strong cell–cell adhesion in either of these cultures.