Figure 2.

The human zw10 transcript and HZW10 gene product. (A) Human zw10 cDNA hybridizes to a single 2.9-kb transcript on a Northern blot of RNAs from three cancer cell lines. Lanes 1–3 contain 2 μg of poly A⁺ RNA from promyelocytic leukemia HL-60, HeLa S3, and chronic myelogenous leukemia K-562 cell lines, respectively. RNA size marker bands are shown at the left. The lower part of the figure shows the same blot probed with actin as a loading control. (B) Western blots of HeLa cell protein extracts were analyzed with HZW10 affinity-purified antibody. Left blot: (lane 1) whole extracts from untransfected cells; (lane 2) whole extracts from cells transfected with a GFP/HZW10 construct. Right blot: HZW10 protein in soluble and insoluble fractions of HeLa cell extracts made in the presence (+) or absence (−) of 1% Triton X-100. The positions of protein standards (E. coli β-galactosidase, 120 kD; rabbit fructose-6-phosphate kinase, 76 kD; and chicken pyruvate kinase, 66 kD) are shown at the left.