Figure 4.
HZW10 immunolocalization in metaphase-arrested HeLa cell chromosome spreads. (A–C) Chromosome spread of a HeLa cell stained with HZW10 (red) and centromeres stained by NR autoimmune CREST serum (green). (D–F) Chromosome spread stained with HZW10 (red), and kinetochores stained by CENP-E (green). DNA is stained in blue in A, B, D, and E. (Arrows) Chromosomes enlarged in the insets. Bar, 5 μm.