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. 1998 Jan 26;140(2):409–418. doi: 10.1083/jcb.140.2.409

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Effects of DN-BMPRs on GAG synthesis in US and LS chondrocyte cultures. US (A) and LS (B) chondrocytes were infected with DN-BMPR viruses, harvested by trypsinization on day 7 (US) or day 5 (LS), and then replated in 24-well plates at the density of 5.0 × 10⁴ cells/dish. Sulfated GAG and DNA contents were determined on day 5 as described in Materials and Methods. rhBMP-2 was added from day 1 at the concentration of 200 ng/ml. Data represent average ± SD for three cultures, and similar results were obtained in two independent experiments.