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. 1998 Jan 26;140(2):409–418. doi: 10.1083/jcb.140.2.409

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Histochemical analysis of proteoglycan accumulation and APase activity in US chondrocytes infected by DN-BMPR viruses. US chondrocytes were infected with RCAS virus encoding DN-BMPRs or vector alone (RCAS) and replated on type I collagen–coated 96-well plates at the density of 2.0 × 10⁴ cells/ dish on day 7. Cells were cultured in the presence of 10 μg/ml of ascorbic acid with (BMP (+)) or without (BMP (−)) rhBMP-2 (200 ng/ml) for 7 d. Cultures were then fixed with 3.7% formaldehyde and stained with Alcian blue (lanes 1 and 4) or crystal violet (lanes 3 and 6) as described in Materials and Methods. Lanes 2 and 5 show the results of histochemical detection of APase.