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. 1998 Jan 26;140(2):409–418. doi: 10.1083/jcb.140.2.409

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Effects of DN- BMPRs on US chondrocytes in serum-free cultures. US chondrocytes were infected with RCAS virus encoding DN-BMPRs or vector alone (RCAS) and replated in type I collagen–coated 96-well plates at the density of 1.0 × 10⁴ cells/dish on day 7. Cells were then cultured in chemically defined medium as described in Materials and Methods for 7 d. The DNA content (A), sulfated GAG content (B), and APase activity (C) were measured as described in Materials and Methods. The data represent average ± SD for three cultures. * unit: One unit of APase activity corresponds to the hydrolysis of 1 nmol per min.