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. 1998 Jan 26;140(2):377–390. doi: 10.1083/jcb.140.2.377

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Cell cycle–dependent localization of GFP-Kar9p. The cell cycle position of a wild-type strain expressing GFP-Kar9p (MS4387) was estimated based on bud size and nuclear morphology. The GFP localization pattern was then determined in unbudded or G1 cells (A–C), small-budded to medium-budded cells or S to M phase cells (D–F), anaphase (G–I), and telophase (J–L) cells. A rare telophase cell with an abnormal localization pattern is shown in panel M. Minor localization patterns were found to be a subset of these six dots. The largest subsets of minor localization patterns were those of two and three dots. GFP: A, D, G, J, and M. DAPI: B, E, H, K, and N. Nomarski: C, F, I, L, and O.