Table V.

Viability of kar9Δ Double Mutants

		Viability with kar9Δ		Tetrads (PD:TT:NPDs)		Predicted double mutants		Percent of double mutants dead or forming microcolonies
dhc1Δ		not viable		32 (2:25:5)		35		100
jnm1Δ		not viable		17 (4:12:1)		14		100
act5Δ		not viable		23 (7:12:4)		20		100
bik1Δ		not viable		16 (3:10:3)		16		94
smy1Δ		viable		8 (0:6:2)		10		0
cin8Δ		viable		15 (2:8:5)		18		33*
kip1Δ		viable		7 (0:7:0)		7		0
kar3Δ		viable		22 (6:13:3)		15‡		NA§

Double mutants were created by crosses. 2–3 d after germination, plates were scored for colony size and the presence of microcolonies. The following crosses were carried out to create the corresponding kar9Δ double mutants. kar9Δ dhc1Δ MS4262 × MS4589. kar9Δ act5Δ MS4586 × MS4263. kar9Δ jnm1Δ MS4321 × MS4587. kar9Δ bik1Δ, MS4734 × MS4313. kar9Δsmy1Δ, APY4ΔD5 × MS4062. kar9Δ cin8Δ, MAY2058 × MS4306. kar9Δ kip1Δ, MS2305 × MS4263. For the analysis of kar9Δ kar3Δ double mutants, MS1614, which carries KAR3 on a URA3-marked CEN plasmid was crossed to the KAR9 delete strain (MS4306). This yielded 15 double mutants carrying the KAR3 URA3 CEN plasmid. The ability of the double mutant to lose the KAR3 plasmid and grow was assayed on plates containing 5-fluoro-orotic acid plates. The ability of the double mutant to lose the KAR3 plasmid was not significantly different in comparison to that of the kar3Δ single mutants. NA, not applicable.  

^* This is the same rate found for single cin8Δ mutants.  

^§ Plasmid loss assay on media containing 5-fluoro-orotic acid was used for the analysis of KAR3.  

^‡ This represents the number of kar9Δ kar3Δ double mutants that carried the KAR3 URA3 plasmid.