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. 1998 Jan 26;140(2):431–446. doi: 10.1083/jcb.140.2.431

Figure 1.

Figure 1

Figure 1

Figure 1

Generation of CD44 glycovariants. (A) Ldl-D cells lack 4-epimerase. The reversible N- and O-linked glycosylation defect in ldl-D cells is due to the lack of a functional 4-epimerase. Culture medium supplementation with Gal and/or GalNAc reverse the N- and O-linked glycosylation defects, respectively. (B) Ldl-D oligosaccharide structures. GalNAc is required to initiate O-linked glycosylation, which is absent without GalNAc supplementation. Gal is required to complete the terminal modifications of complex-type N-linked oligosaccharides and many O-linked oligosaccharides. Truncated N- and O-linked oligosaccharide structures are generated in the absence of Gal supplementation. (C) Glycans after mannosidase inhibition and glycosidase treatment. High mannose–type N-linked oligosaccharides are generated in the presence of dMM and KIF, metabolic inhibitors of α mannosidase I. Hybrid type N-linked oligosaccharides are generated in the presence of swainsonine, an α mannosidase II inhibitor. The oligosaccharide structures remaining on CD44Rg after Endo H digestion of high mannose–type N-linked oligosaccharides or PNGase-F digestion are shown.