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. 1998 Jan 26;140(2):431–446. doi: 10.1083/jcb.140.2.431

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Effect of glycosylation on the binding of soluble HA to CHO or ldl-D cells. HA binding to ldl-D cells was assessed by FACS^® analysis performed using the indicated concentrations of FITC-HA, and CHO or ldl-D cells that were cultured for 4 d with the indicated saccharide additions. HA binding was performed after preincubation of cells with blocking anti-CD44 antibody KM-81 (dotted line) or an isotype-matched unrelated antibody (solid line). The same results were obtained from four other experiments.