Table I.
Inhibition of HIV-1–mediated Cell–Cell Fusion by DP-178 Peptide
| Peptide concentration | (A) β-Galactosidase activity | (B) DiI/DiO transfer | ||
|---|---|---|---|---|
| ng/ml | % | % | ||
| 0 | 100 | 100 | ||
| 0.5 | 109 ± 12.7 | 104.5 ± 4.5 | ||
| 2.5 | 49.05 ± 1.3 | 79.6 ± 6.4 | ||
| 5 | 24.4 ± 3.4 | 41.5 ± 16.4 | ||
| 25 | 12.5 ± 2.7 | 34.9 ± 9.6 | ||
| 50 | 1.15 ± 0.8 | 31.7 ± 9.1 | ||
| 100 | N.D. | 20.5 ± 4.0 | ||
| 500 | 2.5 ± 3.6 | N.D. |
Values were calculated as mean ± SD of two independent experiments in A, and of three experiments in B. For data in A, β-galactosidase activity was detected according to the gene reporter assay. After 2 h of incubation in 96-microwell plates, the percentage of cell–cell fusion was calculated from the enzymatic activity of the control (0 ng/ ml of peptide). For data in B, TF228 cells were labeled with DiI, and SupT1 cells with DiO; percentage of cell–cell fusion was calculated from the control as described in the text. N.D., not determined.