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. 1998 Jan 26;140(2):355–366. doi: 10.1083/jcb.140.2.355

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Phenotype of CYK1 gene disruption. (A) Tetrad analysis of a diploid strain (RLY226) heterozygous for the Δcyk1 mutation showing 2:2 segregation of viability. The plate was photographed after 5-d growth at 23°C. (B) The morphology of a typical Δcyk1 microcolony after 24-h growth at 23°C, after tetrad dissection. The microcolony did not further increase in size and the cells eventually lysed. The photograph was taken under a Zeiss Axiophot microscope with a ×40 Nikon 0.5 ELWD objective. Bar, 20 μm.