Figure 2.

Influence of spatial match threshold. Three hundred randomly rotated particle projections of each of 300 cluster configurations were simulated with ±3.5-Å random noise added and aligned using a range of spatial match thresholds. The percentages of correctly matched particles, unmatched particles, and ambiguous particles at each threshold are shown, while the final category (mistakenly matched particles), which makes up the remaining percentage, is not shown. In addition, the same number of particles, deformed to model a 10° domain shift, were also subjected to the alignment procedure, and the percentage included (eluding detection) is shown.