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. 1998 Jan 12;140(1):81–90. doi: 10.1083/jcb.140.1.81

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Protein and PLD activity of Spo14 variants. (A) Immunoblot blot analysis of immunoprecipitates from cells harboring SPO14 CEN (lane Spo14p; Y501), HA-SPO14 CEN (lane HA-Spo14p; Y568), HA-spo14-ΔN CEN (lane HA-Spo14p^ΔN; Y951), HA-spo14-K→ H CEN (lane HA-Spo14p^{K→ H}; Y795), HA-SPO14 2μ (lane HA-Spo14p 2μ; Y602), and HA-spo14-ΔN 2μ (lane HA-Spo14p^ΔN 2μ; Y1031). The numbers on the left of the immunoblot indicate the positions of molecular mass standards (kD). (B) Sporulation (white bars) and total PLD activity (hatched bars) and relative PLD specific activity (black bars) of the corresponding proteins. PLD assays were performed with vesicles containing 100 μM BODIPY-PC and 5 μM PIP₂.