Table I.
Yeast Strains
| Strain | Genotype | Source | ||
|---|---|---|---|---|
| KR52-3C | HOura3-1leu2arg4-8 thr1-4trp1-1ade2spo14::URA3 | Rose et al., 1995 | ||
| HO ura3-1 leu2 arg4-8 thr1-4 trp1-1 ade2 spo14::URA3 | ||||
| Y501 | KR52-3C, plus pME865 (SPO14 LEU2 CEN) | This study | ||
| Y568 | KR52-3C, plus pME940 (HA-SPO14 LEU2 CEN) | This study | ||
| Y951 | KR52-3C, plus pME1132 (HA-spo14ΔN LEU2 CEN) | This study | ||
| Y795 | KR52-3C, plus pME1043 (HA-spo14-K→ H LEU2 CEN) | This study | ||
| Y603 | KR52-3C, plus pME962 (SPO14 LEU2 2μ) | This study | ||
| Y602 | KR52-3C, plus pME957 (HA-SPO14 LEU2 2μ) | This study | ||
| Y1031 | KR52-3C, plus pME1121 (HA-spo14-ΔN LEU2 2μ) | This study | ||
| NH144 | MATa leu2-k HIS4 ho:: LYS2 ura3 lys2 arg4-Nsp | Hollingsworth et al., 1995 | ||
| MATα leu2::hisG his4-x ho::LYS2 ura3 lys2 ARG4 | ||||
| Y433 | NH144 but homozygous spo14::URA3 | This study | ||
| Y969 | Y433, plus pME1096 (GFP-SPO14 LEU2 2μ) | This study | ||
| Y988 | NH144, plus pME1124 (GFP-spo14-ΔN LEU2 2μ) | This study | ||
| Y989 | Y433, plus pME1124 (GFP-spo14-ΔN LEU2 2μ) | This study | ||
| Y1019 | NH144, plus pME1130 (GFP-spo14-K→ H LEU2 2μ) | This study | ||
| Y1020 | Y433, plus pME1130 (GFP-spo14-K→ H LEU2 2μ) | This study |