Figure 5.

A mutation of the p130^Cas binding site on FAK does not affect its association with Src. Cell lysates were prepared from a control clone (Neo) or from clones expressing either wild-type (WT), Y397F, or P712/715A FAK, as indicated. Total cell lysate proteins were Western blotted with KT3 to demonstrate relative levels of exogenous FAK expression (A). Alternatively, Src was immunoprecipitated from the lysates using the anti-Src mAb 2-17, and the immune complexes were Western blotted with KT3 to demonstrate Src-associated FAK (B).