Figure 9.

Expression of p130^Cas enhances FAK-promoted cell migration. Cell lysates were prepared from a clone expressing FAK (WT), three clones expressing both FAK and wild-type p130^Cas (WT/Cas-1, 2, and 3), or a control clone (Neo), as indicated. Total cell lysate proteins were Western blotted with mAb KT3 to demonstrate exogenous FAK expression (A) or mAb 9E10 to demonstrate exogenous p130^Cas expression, which is indicated by an arrow (B). Molecular mass positions (kD) are shown on the right (B). Migration of the above clones was analyzed using a chemotaxis chamber and 3 μg/ml FN as an attractant (C). Mean cell counts from at least 20 fields and three experiments are shown. Error bars represent standard errors.