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. 1997 Dec 15;139(6):1419–1431. doi: 10.1083/jcb.139.6.1419

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Location of AOX protein in P. pastoris wild-type (WT) and selected pex strains. Methanol-induced cells of each strain were spheroplasted, osmotically lysed, and subjected to differential centrifugation. Protein samples of 30 μg from the resulting organelle pellet (P) and cytosolic supernatant (S) fractions were subjected to SDS-PAGE and immunoblotting with anti-AOX antibodies.