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. 1997 Dec 15;139(6):1419–1431. doi: 10.1083/jcb.139.6.1419

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Analysis of octameric AOX in the P. pastoris strain coexpressing AOX–HA and AOX-WT. (A) Octameric AOX (fraction 9 from the sucrose velocity gradients shown in Fig. 6) was immunoprecipitated using HA-tag monoclonal antibodies, subjected to SDS-PAGE, and immunoblotted using antibodies against AOX (top) and HA (bottom). The SDS-PAGE lanes were loaded with equal ratios (relative to top and bottom) of sample from the following strains: Lane 1, AOX–HA; lane 2, AOX-WT/AOX–HA; lane 3, nonimmunoprecipitated AOX-WT, and lane 4, AOX-WT. (B) Octameric AOX from fraction 9 of the gradients in Fig. 6 were subjected to electrophoresis on a 5% native (nonreducing, nondenaturing) polyacrylamide gel and immunoblotted using antibodies against AOX (top) and HA (bottom). Lane 1, AOX-WT; lane 2, AOX-WT/AOX-HA; lane 3, AOX–HA; and lane 4, a mixture of fractions from both the AOX–HA strain and the AOX-WT/AOX–HA strain.