Table I.
Yeast Strains
| Strain | Genotype | Source or reference | ||
|---|---|---|---|---|
| P. pastoris | ||||
| Wild type | NRRL Y-11430 | |||
| GS115 | his4 | Cregg et al., 1985 | ||
| GS-HWOxx | GS115 transformed with plasmid pHWOxx. | This study | ||
| MC100-3 (aoxΔ) | aox1Δ::ScARG4 aox2Δ::Pphis4 arg4 his4 | Cregg et al., 1989 | ||
| MC-HWOxx | MC100-3 transformed with plasmid pHWOxx. | This study | ||
| MC200 (pex2Δ aoxΔ) | pex2Δ::ScHIS4 aox1Δ::ScARG4 aox2Δ::Pphis4 arg4 his4 | This study | ||
| MC200-MOX | aox1Δ::ScARG4 aox2Δ::Pphis4 pex2Δ::ScHIS4 arg4 his4 (pHWO56) | This study | ||
| JC116 (pex2-1) | pex2-1 his4 (formerly per6-1) | Waterham et al., 1996 | ||
| JC117 (pex2Δ) | pex2Δ::PpARG4 arg4 his4 | This study | ||
| JC120 (pex8-1) | pex8-1 his4 (formerly per3-1) | Liu et al., 1995 | ||
| JC125 (pex8Δ) | pex8Δ::ScARG4 arg4 his4 (formerly per3Δ) | Liu et al., 1995 | ||
| pex5Δ | pex5Δ::PpARG4 arg4 his4 (formerly pas8Δ) | McCollum et al., 1993 | ||
| pex5Δ-HWOxx | pex5Δ strain transformed with plasmid pHWOxx. | This study | ||
| H. polymorpha | ||||
| A16 | leu1.1 | Veale et al., 1992 | ||
| CT100 (pex10) | pex10-1 leu1.1 (formerly per8-1) | Tan et al., 1995a | ||
| CW110 (pex10 moxΔ) | pex10-1 moxΔ::ZEO leu 1.1 | This study | ||
| CW111 (pex10-1 moxΔ pex10-1 moxΔ [pHWO57] AOX) | This study | |||
| S. cerevisiae | ||||
| SFY526 | MATa, ura3-52, his3-200, ade2-101, lys2-801, | Bartel et al., 1993 | ||
| trp 1-901, leu2-3, 112, canr, gal4-542, gal80-538, | ||||
| URA3::GAL1-lacZ |