Table II.
Plasmids
| Plasmid | Relevant properties | Reference | ||
|---|---|---|---|---|
| P. pastoris | ||||
| pHIL-A1 | Apr, PpHIS4, PAOX1,tAOX1 | Waterham et al., 1997 | ||
| pHWO40 | Apr, PpHIS4, PAOX1-AOX-WT (M1-F663)-tAOX1 | This study | ||
| PHWO41 | Apr, PpHIS4, PAOX1-AOX-RSC (M1-G659RSC)-tAOX1 | This study | ||
| pHWO42 | Apr, PpHIS4, PAOX1-AOX-HA (M1-G659YPYDVPDYAG)-tAOX1 | This study | ||
| pHWO43 | Apr, PpHIS4, PAOX1-AOX-Δ22 (M1 -A642)-tAOX1 | This study | ||
| pHWO10 | Apr, PpHIS4, PGAP, tAOX1 | Waterham et al., 1997 | ||
| pHWO10K | Kmr, PpHIS4, PGAP, tAOX1 | This study | ||
| pHWO19 | Kmr, PpHIS4, PGAP-β-lac (MSGH24-W286)-tAOX1 | Waterham et al., 1997 | ||
| pHWO44 | Kmr, PpHIS4, PGAP-β-lac-AOX642-663 (MSGH24-W286SRV-A642-F663)-tAOX1 | This study | ||
| pHWO45 | Kmr, PpHIS4, PGAP-β-lac-AOX660-663 (MSGH24-W286SRV-G659-F663)-tAOX1 | This study | ||
| pHWO46 | Kmr, PpHIS4, PGAP-β-lac-AOX642-659-RSC (MSGH24-W286SRV-A642-G659RSC)-tAOX1 | This study | ||
| pHWO47 | Kmr, PpHIS4, PGAP-β-lac-AOX642-659-HA (MSGH24-W286SRV-A642-G659YPYDVPDYAG)-tAOX1 | This study | ||
| pUZ12 | Apr, pex2Δ::ScHIS4 | Waterham et al., 1996 | ||
| pPICZ-B | ZEO, PAOX1, tAOX1 | Invitrogen | ||
| pHWO55 | ZEO, PAOX1, PMOX-MOX, tAOX1 | This study | ||
| pHWO56 | Apr, PpHIS4, PAOX1, PMOX-MOX, tAOX1 | This study | ||
| S. cerevisiae | ||||
| pGBT9 | Apr, ScTRP1, PADH1-GAL4B | Clontech | ||
| pHWO48 | Apr, ScTRP1, PADH1-GAL4B-AOX642-663 (E881IEFPA642-F663) | This study | ||
| pHWO49 | Apr, ScTRP1, PADH1-GAL4B-AOX642-663-RSC (E881IEFPA642-G659RSC) | This study | ||
| pHWO50 | Apr, ScTRP1, PADH1-GAL4B-AOX642-659-HA (E881IEFPA642-G659YPYDVPDYAG) | This study | ||
| pGAD424 | Apr, ScLEU2, PADH1-GAL4A | Clontech | ||
| pHWO51 | Apr, ScLEU2, PADH1-GAL4A-PEX5 (S147PEFPGIRG13-F576) | This study | ||
| pUT352 | Apr, ScURA3, ZEO | Wenzel et al., 1992 | ||
| H. polymorpha | ||||
| pMOX-BS | Apr, pUC18 + BamHI–SacI MOX fragment | Distel et al., 1987 | ||
| pET1 | Apr, ScLEU2, PMOX | Tan et al., 1995b | ||
| pHWO52 | Apr, moxΔ::ZEO | This study | ||
| pHWO57 | Apr, PpHIS4, ScLEU2, PAOX1-AOX-tAOX1 | This study | ||
| pHWO58 | Apr, ScLEU2, PMOX-AOX-tAOX1 | This study |
Bold, underline, and capital; PpAOX1 amino acid sequence numbered according to Koutz et al. (1989). Bold, italic, and capital; β-lactamase amino acid sequence numbered according to Sutcliffe (1978). Bold and capital; Pex5p amino acid sequence numbered according to McCollum et al. (1993).