Table III.

Peroxisomal Enzyme Activities in Organelle Pellet and Cytosolic Supernatant Fractions of Methanol-induced P. pastoris pex strains

Strain		Fraction*		AOX‡		CAT§		Cyt c ox.‡		GAPDH‡
Wild type		P		0.35		33		1.9		UD
		S		0.33		32		0.0041		0.21
		%P§		51		51		99		0.0
pex2-1 (JC116)		P		0.0031		1.9		1.4		0.011
		S		UD		20		0.014		0.28
		%P		100		8.7		99		3.8
pex2Δ (JC117)		P		UD		3.5		1.4		0.010
		S		UD		72		UD		0.32
		%P		—		4.6		100		3.0
pex8-1 (JC120)		P		0.011		3.3		1.5		0.0050
		S		0.00070		45		0.0097		0.19
		%P		94		6.8		99		2.6
pex8Δ (JC125)		P		UD		3.4		1.1		0.060
		S		UD		44		UD		0.32
		%P		—		7.2		100		16

^*  P and S are enzyme activities in organellar pellet and cytosolic supernatant fractions, respectively. %P is percentage of enzyme activity in the pellet fraction.  

^‡ Activities are expressed as U/mg protein.  

^§ Activity is expressed as ΔE240/min per mg protein. UD indicates activity is undetectable.