Table V.

Peroxisomal Enzyme Activities in Organelle Pellet and Cytosolic Supernatant Fractions of Methanol-induced P. pastoris Strains Expressing Mutant AOX1 Genes

Strain		t  1/2		Fraction*		AOX‡		CAT§		Cyt c ox‡		GAPDH‡
AOX-WT		3.5		P		0.54		74		5.3		0.077
(MC-HWO40)				S		0.38		65		0.51		2.3
				%P		59		53		99		3.2
AOXΔ22		>100		P		UD		56		0.59		0.067
(MC-HWO43)				S		UD		19		0.0052		2.0
				%P		—		86		99		3.2
AOX–RSC		18		P		0.56		71		4.5		0.36
(MC-HWO41)				S		0.025		29		0.055		2.4
				%P		69		71		99		13
AOX–HA		22		P		0.010		47		7.0		0.60
(MC-HWO42)				S		UD		20		0.043		4.9
				%P		100		70		99		11
AOX+AOX–HA		4.3		P		0.38		94		7.5		0.083
(GS-HWO42)				S		0.28		74		0.030		1.5
				%P		58		56		99		5.2

^*  P and S are enzyme activities in organellar pellet and cytosolic supernatant fractions, respectively. %P is percentage of enzyme activity in the pellet fraction.  

^‡ Activities are expressed as U/mg protein.  

^§Activity is expressed as ΔE240/min per mg protein. UD indicates activity is undetectable.