Figure 1.

Receptor-induced liposome binding. Biotinylated PI-PLC–released API was incubated with or without sTva for 15 min at 4°C incubated with liposomes at 37°C for 30 min and then analyzed on flotation gradients as described in Materials and Methods. Gradients were fractionated, and samples were immunoprecipitated with anti-DAF antibody, boiled and reduced, separated by SDS-12.5% PAGE, transferred to nitrocellulose, probed with streptavidin-HRP, and visualized by ECL to identify API.