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. 1997 Dec 15;139(6):1455–1464. doi: 10.1083/jcb.139.6.1455

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Liposome binding capability of Glu2-PI. The position 2 glutamate mutant protein (Glu2-PI) was assayed for liposome binding as in Fig. 4. Fractions were immunoprecipitated with anti-DAF, processed by SDS-12.5% PAGE and transferred to nitrocellulose. Blots were probed with (A) streptavidin-HRP (visualized by enhanced chemiluminescence), or (B) ¹²⁵I-labeled streptavidin (quantitated by phosphorimager analysis as described in Materials and Methods). (% Bound) Percent API or Glu2-PI in the top three fractions.

Liposome binding capability of Glu2-PI. The position 2 glutamate mutant protein (Glu2-PI) was assayed for liposome binding as in Fig. 4. Fractions were immunoprecipitated with anti-DAF, processed by SDS-12.5% PAGE and transferred to nitrocellulose. Blots were probed with (A) streptavidin-HRP (visualized by enhanced chemiluminescence), or (B) ¹²⁵I-labeled streptavidin (quantitated by phosphorimager analysis as described in Materials and Methods). (% Bound) Percent API or Glu2-PI in the top three fractions.