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. 1997 Dec 15;139(6):1383–1396. doi: 10.1083/jcb.139.6.1383

Figure 13.

Figure 13

Formation of the extended CBF3 complex on probes containing wild-type and inverted CDEII sequences. (a) rCBF3 on its own or mixed with excess p110 (as indicated) was bound to an 89-bp wild-type probe (lanes 1 and 2), an 89-bp probe containing a linker to the right of base +56 (lanes 3 and 4), or an 89-bp probe containing 33 bp of CDEII to the left of base +1 (lanes 5 and 6) and analyzed on a bandshift gel. (b) rCBF3 was mixed with a radiolabeled 56-bp inverted-CDEIII DNA probe (lane 7) or radiolabeled 106-bp inverted-CDEIII DNA probe (lanes 8–10) and analyzed on a bandshift gel. Specificity was demonstrated by the addition of wild-type or mutant CDEIII DNA competitors, as indicated.