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. 1997 Dec 15;139(6):1383–1396. doi: 10.1083/jcb.139.6.1383

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UV crosslinking of rCBF3 to the bottom strand of radiolabeled 56-bp CDEIII DNA. (a) rCBF3 proteins labeled by DNA crosslinking probes (as indicated) were run on an SDS-containing gel. The positions of molecular weight markers are shown. The types of crosslinking probe and CBF3 preparation used in each lane are indicated above the gel. The specificity of CBF3 binding was tested by including a 200-fold excess of unlabeled wild-type (WT; probe BC1) or mutant CDEIII competitor (Mut; probe BC2) DNA in the binding reaction. Some reactions contain rCBF3 with p58 (tag-p58) or p64 (tag-p64) proteins that are linked at their amino termini to a short epitope tag. p110Δ refers to rCBF3 containing p110 protein with a carboxy-terminal 122 amino acid deletion. For a complete description of probe X1 see Figs. 1 and 3. (b) Crosslinking reactions containing recombinant p64/p58/23 (lane 9), recombinant p110 protein (lane 10), or a mixture of these two extracts (lane 11) was analyzed as above. (c) One fifth of each crosslinking reaction from a was analyzed on bandshift gels (lanes 12–19). (d) One fifth of each crosslinking reaction from b was analyzed on bandshift gels (lanes 20–22).