Skip to main content
. 1997 Dec 15;139(6):1383–1396. doi: 10.1083/jcb.139.6.1383

Figure 6.

Figure 6

Localization of protein–DNA crosslinks on the bottom strand of 56-bp CDEIII DNA. (a) The site-specific crosslinkers, BrdC or BrdU, were incorporated at the positions indicated into the bottom strand of DNA. In each case “none” refers to the synthesis of probe in the absence of BrdC or BrdU. (b) Site-specific BrdC crosslinkers (indicated by asterisks) were incorporated using mutant top-strand oligos and appropriate bottom-strand oligos by enzymatic extension (see Fig. 1 for details about the probes). Crosslinking reactions using probes described in a and b were analyzed on SDS-containing gels as in Fig. 4. Faint labeling of p58 in lanes 18–20 are a result of non-BrdC–dependent binding as in Fig. 5, lane 12.