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. 1997 Dec 15;139(6):1383–1396. doi: 10.1083/jcb.139.6.1383

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UV crosslinking of rCBF3 to progressively shorter labeled regions of the top strand of the 56-bp CDEIII DNA. (a) BrdU was incorporated into each crosslinking probe as in Fig. 5. Reactions were carried out in the presence of either wild-type or tagged CBF3 proteins and analyzed on an SDS-containing gel. (b) Probes with either BrdU or BrdC incorporated into the entire top strand (+2 to +56; lanes 11 and 12) or a probe with BrdC incorporated only to the right of the CCG (+22 to 56; lane 13) were mixed with rCBF3 and analyzed on bandshift gels.