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. 1997 Dec 15;139(6):1383–1396. doi: 10.1083/jcb.139.6.1383

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Analysis of centromeres containing point mutations in sequences that crosslink to CBF3 subunits. (a) rCBF3 and radiolabeled probe BC4 were mixed with a 4- to 200-fold excess of unlabeled centromeric DNA competitors (as indicated) and analyzed on bandshift gel. Values are expressed as a percentage of core complex CDEIII binding observed in the absence of competitor. Error bars represent the range of values obtained in duplicate experiments. (b) rCBF3 was mixed with radiolabeled 56-bp wild-type DNA (Probe BC4; lane 1), linker-modified 56-bp DNA (Probe BC5; lane 2), or 38-bp probe DNA (Probe BC6; lane 3), and CDEIII binding was analyzed on bandshift gels.