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. 1997 Dec 15;139(6):1433–1446. doi: 10.1083/jcb.139.6.1433

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Localization of Ii and HLA-DM by immunofluorescence microscopy. (a) Cells grown on coverslips were fixed, permeabilized and labeled for Ii using anti-Ii NH₂-terminal monoclonal (a) or polyclonal (d) antibodies, and HLA-DM using polyclonal (b) or monoclonal antibodies (e) followed by FITC (b and d) or Texas red (a and e) conjugated secondary antibodies. The superposition of the images is shown in c and f. In b, cells were labeled for MHC class II molecules using monoclonal (b) or polyclonal (e) antibodies and double labeled for Ii using polyclonal anti-Ii NH₂-terminal antibodies (a) or HLA-DM using mAbs (d), followed by Texas red (b and e) or FITC (a and d) conjugated secondary antibodies. c and f show the merged images. Bars: 20 μm.