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. 1997 Dec 15;139(6):1477–1484. doi: 10.1083/jcb.139.6.1477

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ET-1 increases expression of sarcomeric MyHC in myofibroblasts. (A) Indirect immunofluorescence was performed to determine the number of sarcomeric MyHC-positive liver stellate cells and BHK cells before (−) and after (+) treatment with 10⁻¹⁰ M ET-1 for 24 h. (B) Immunoblotting was performed to determine the increase of sarcomeric MyHC in liver stellate cells and BHK cells in the absence of serum (lane 1), in the presence of 10% serum (lane 2), in the presence of 5 × 10⁻⁷ M ET-1 antagonist (lane 3), and in the presence of 10⁻¹⁰ M ET-1 for 24 h (lane 4).