Table I.
Muscle Protein Expression in Myofibroblasts*
| In Vivo‡ | Liver stellate cells | BHK cells | L cells | |||||
|---|---|---|---|---|---|---|---|---|
| Sarcomeric MyHC | + | + | − | |||||
| Embryonic | − | + | − | |||||
| Perinatal | + | + | − | |||||
| Adult skeletal fast IIa | + | + | − | |||||
| Adult skeletal fast IIb | − | + | − | |||||
| Adult skeletal fast IId | + | + | + | − | ||||
| α-Cardiac MyHC | − | − | − | |||||
| β MyHC | − | + | − | |||||
| Sarcomeric MLC | − | + | − | |||||
| Sarcomeric actin | − | + | − | |||||
| Sarcomeric tropomyosin (α,β) | − | + | − | |||||
| Sarcomeric troponin T | − | + | − | |||||
| Smooth-muscle MyHC | + | + | + | − | ||||
| Smooth-muscle actin | + | + | + | − | ||||
| Titin | − | − | − | |||||
| Desmin | + | + | + | − |
*
Cells were grown on coverslips under normal growth conditions. Coverslips were fixed and stained as described in Materials and Methods. 100-mm dishes were scraped in Laemmli sample buffer. Lysate was used for immunoblotting.
‡
Previous in vivo reports in myofibroblasts (Benzonana et al., 1988; Sappino et al., 1990; Ogata et al., 1993).