Figure 3.
The redistribution of IBV M induced by PDMP was rapid and reversible. BHK-21 cells infected with a recombinant vaccinia virus encoding IBV M were treated 4 h after infection with 50 μg/ml cycloheximide for 1 h. PDMP (100 μM) was added, and dishes were fixed at the indicated times and prepared for indirect immunofluorescence. After 1 h in PDMP, the remaining dishes were washed three times with 1 ml DME with serum and incubated with cycloheximide-containing medium for the indicated time before being prepared for indirect immunofluorescence. For each experimental series, the Nomarski image is shown on the left and the fluorescence image on the right. Bar, 10 μm.