Figure 7.

Effect of pretreatment with βCA and FB1 on PDMP-induced redistribution of IBV M. Cells were prepared for immunofluorescence as described in Fig. 2 with the exception that indicated samples were treated with either 5 mM βCA or 100 μM FB1 beginning with the cycloheximide chase 4 h after infection. At 5 h after infection, cells were treated with 1% isopropanol (control) or 100 μM PDMP. 1 h later the cells were fixed and prepared for indirect immunofluorescence. Random fields from coded samples were scored for the number of IBV M–expressing cells that fell into the three classes of staining pattern shown. Treating cells with FB1 or βCA before PDMP shifted the IBV M staining pattern towards the control pattern.