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. 1997 Dec 29;139(7):1597–1610. doi: 10.1083/jcb.139.7.1597

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Analysis of replication labelling patterns in metaphase chromosomes. Metaphase smears were obtained from cells pulsed with BrdU at Stages 1–5 of S phase. BrdU staining and DAPI staining of the same chromosomes is shown side by side. Stage 1 and 2: major R bands show intense BrdU signal (large arrowheads). Stage 3: BrdU signal concentrates in Q bands (large arrowheads). Stage 4: BrdU signal concentrates in Q bands (large arrowheads) that spread along the length of every chromosome as shown in the whole chromosome complement from a single cell (larger panels). Stage 5: BrdU staining is restricted to a few bands per chromosome. Note that a prominent R band from a stage 2 chromosome (small arrowhead) is devoid of BrdU signal and that, in a stage 4 chromosome, an R band region (small arrowhead) is BrdU positive. Bar, 3 μm.