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. 1997 Dec 29;139(7):1597–1610. doi: 10.1083/jcb.139.7.1597

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Chromatin replication patterns in PtK cells. PtK cells were pulse labelled with BrdU immediately before harvesting. BrdU incorporation sites were visualized by indirect immunofluorescence (A–D) and total DNA was stained with YOYO (A′– D′). Shown are optical sections through nuclei considered representative of each pattern. (A) Pattern A, (B) pattern B, (C) pattern C, (D) pattern D. The temporal order of replication patterns in PtK cells was established by pulse labelling asynchronous cultures with CldU (5 μM; 30 min) followed 3–5 h later by a second pulse with IdU (2:5 μM; 30 min). In the cell shown (E and F), the two pulses were administered 3 h apart, and a succession from pattern B (E) to pattern C (F) is observed. Bar, 10 μm.