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. 1997 Dec 29;139(7):1597–1610. doi: 10.1083/jcb.139.7.1597

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In PtK cells, peripheral chromatin that replicates in the second half of S phase (replication pattern C) is contributed by all chromosomes. Asynchronously growing PtK cells were pulse labelled with BrdU (10 mM; 30 min) and, after release into BrdU-free medium, the cells were harvested at hourly intervals. BrdU-DNA was revealed by indirect immunofluorescence and total DNA by staining with YOYO. For each time point, we scored the percentage of mitotics (prophases) showing “complete” labelling (i.e., BrdU labelling present in all chromosomes) and the percentage of BrdU-labelled interphase cells that displayed pattern C (see text for details). A minimum of 50 mitotic and of 120 interphase cells were analyzed for each time point. The graph shows that during the time period, stage C cells undergo mitosis, as evidenced by the decline in their percentage (thin line), the vast majority of the corresponding mitotic cells (89–100%; thick line) display complete labelling.