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. 1997 Dec 29;139(7):1597–1610. doi: 10.1083/jcb.139.7.1597

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Association of chromosomes with the nuclear periphery. Human lymphoid cells (IM-9 cells) were labelled with BrdU (5 μM) for a full S phase, and then grown in BrdU-free medium for ∼7 d to generate a population with only a few particles labelled. Cells were double immunolabelled for BrdU (green) and lamin B (red) and serial optical sections were obtained by confocal microscopy at 0.4-μM intervals. Complete (A) and partial (B) confocal series are depicted. Note that all labelled particles reach the nuclear periphery. (C) Metaphase spreads were obtained from the same cell population after addition of colcemid to the cultures. Images from total DNA stained with DAPI (red) and BrdU-DNA (yellow) were acquired with a SIT camera and electronically superimposed. Only a few chromosomes show BrdU staining. Bars, 10 μm.