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. 1997 Dec 29;139(7):1597–1610. doi: 10.1083/jcb.139.7.1597

Figure 8.

Figure 8

Micronuclei display features typical of active interphase nuclei. The results shown were obtained with CHF cells induced to micronucleate in the presence of colcemid. (A) Transcriptional activity in micronuclei as assessed by incorporation of Br-UTP (refer to Materials and Methods). The sites of transcription are visualized as multiple foci distributed throughout the nucleoplasm as previously shown for intact nuclei (see for example Wansink et al., 1993; Ferreira et al., 1994). The hnRNP A1 protein was immunolocalized in micronuclei with mAb 4B10 (D). Poly (A) RNA as detected by in situ hybridization with a Poly (U) oligoribonucleotide probe in intact nuclei (B) and in micronuclei (E). Micronuclei efficiently incorporate BrdU (10 μM; 30 min) and show replication patterns similar to those seen in whole nuclei (C, pattern 1 and F, pattern 4). Bar, 10 μm.