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. 1997 Dec 29;139(7):1775–1783. doi: 10.1083/jcb.139.7.1775

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Immunoblots of Triton X-100 extracts from stably transfected L. donovani promastigotes overexpressing the D2 hexose transporter (lanes 1 and 2) or the MIT myo-inositol transporter (lanes 3 and 4). P designates the pellet (lanes 1 and 3) and S designates the supernatants (lanes 2 and 4) of the detergent extracts. Lanes 1 and 2 were stained with the anti-D2 antibody (1:200), and lanes 3 and 4 were stained with the anti-MIT antibody (1:3,000). Numbers to the left indicate the mobilities of protein molecular mass markers in kilodaltons.