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. 1997 Dec 29;139(7):1775–1783. doi: 10.1083/jcb.139.7.1775

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Confocal laser scanning microscopy of Triton X-100 extracted promastigotes of L. donovani overexpressing the MIT myo-inositol transporter (top) or the D2 hexose transporter (bottom). Each cytoskeleton was double-stained with either the anti-MIT antibody (MIT, 1:1,000) or the anti-D2 antibody (D2, 1:200), followed by the control α-tubulin antibody (α-tub, 1:500). Secondary FITC-conjugated or rhodamine-conjugated antibodies were used to reveal staining of the antitransporter or anti–α-tubulin antibodies, respectively. Each micrograph represents a single 0.5-μm section through each field.