Figure 1.

ldlF cells lack ε-COP. After incubation of wt and mutant cells at either 34° or 40°C for 12 h, cell lysates were normalized for total cell protein and then subjected to SDS-PAGE and immunoblotting. At 34°C, ldlF cells have much less ε-COP than wt cells, and after incubation at 40°C for 12 h, ε-COP is undetectable in the mutant cells. The defect was complemented by transfection with wt ε-COP in ldlF[LDLF] cells.