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. 1997 Dec 29;139(7):1747–1759. doi: 10.1083/jcb.139.7.1747

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SFV does not reach an acidic compartment in ldlF cells. After SFV uptake for 30 min, delivery of SFV to an acidic compartment was monitored by immunofluorescence detection of the acid-specific (pH < 6.2) conformation of SFV–E1 protien. Cells were preincubated at 34° or 40°C for 10 h. The acid conformation was detected in ldlF cells preincubated at 34°C, wt, and ldlF[LDLF] cells, but not ldlF cells preincubated at 40°C. wt and ldlF[LDLF] are representative of results obtained at either temperature.