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. 1997 Dec 29;139(7):1747–1759. doi: 10.1083/jcb.139.7.1747

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Endosomal pH is similar in wt, ldlF, and ldlF[LDLF] cells. After incubation of wt and mutant cells at 34° and 40°C for 8 h, cells were loaded with FITC–Tfn. FITC fluorescence was quantitated both before and after equilibration to pH 7.4 with nigericin. The recorded change in fluoresence intensity is proportional to the difference between the pH of the FITC–Tfn-containing endosomes and that of the equilibration buffer (pH 7.4). Within the limits of these experiments, there was no significant difference observed between ldlf mutant cells and either the wt or ldlf[LDLF] mutant cells. n = 8 for wt cells at 40°C, n = 9 for ldlF cells at 40°C, and n = 6 for all others.