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. 1997 Dec 29;139(7):1621–1634. doi: 10.1083/jcb.139.7.1621

Figure 8.

Figure 8

Detection of cytoplasmic Sp100 protein in a coculture of human HuH-7 and rat R1H cells by immunofluorescence analysis. Untreated HuH-7 cells when stained with polyclonal rabbit anti-Sp100 Abs (Sp26) lack ND staining but show cytoplasmic fluorescence (Sp100/Ø). After treatment with IFN, a clear ND staining became visible in addition to the cytoplasmic staining (Sp100/IFN). Both images were taken at the same exposure time. At this exposure, rat R1H cells are not visibly stained by the Abs and serve as an internal negative control. Localization of the rat cells is visible in the corresponding phase contrast pictures given below. Bar, 10 μm.