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. 1997 Dec 29;139(7):1635–1643. doi: 10.1083/jcb.139.7.1635

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Subunit composition of the induced NF-κB–DNA complexes after treatment with LPS (A) or ATP (B). Total cell extracts of non-stimulated (lanes 1) or stimulated (lanes 2) N9 cells were prepared and either left untreated (lanes 1 and 2) or incubated with specific antibodies against the NF-κB subunits p50 (lanes 3), p65 (lanes 4), c-Rel (lanes 5) and RelB (lanes 6). Lanes 7 denote an extract of ATP-treated cells incubated with recombinant IκB-α that releases NF-κB–specific complexes from bound DNA. Only sections of the autoradiograms are shown.