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. 1997 Dec 29;139(7):1735–1745. doi: 10.1083/jcb.139.7.1735

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Rapid turnover of furin is not mediated by the transmembrane and cytosolic domains. Stably transfected RBL cells expressing Tac, T-T-F, T-F-F, or furin-FLAG (see Fig. 1 for structures) were pulse labeled with [³⁵S]methionine for 30 min and chased for 0, 3, or 6 h. Tac species were isolated by immunoprecipitation with the monoclonal antibody 7G7 and furin species with a mixture of Fur1, Fur2, and DC16. The symbols p and m point to the ER precursor and Golgi-processed, mature forms of the Tac proteins, respectively. The positions of molecular weight markers (expressed as 10⁻³ × M _r) are shown at right.